Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Table 4 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector during fed-batch culture

Clone Name	Culture period (day)	Relative composition of monosaccharide			Fucose(-)%^b
		Fucose^a	GlcNAc	Mannose
32-05-12	6	0.92	4.00	2.86	8
	12	0.94	4.00	2.99	6
	16	0.97	4.00	3.01	3
FG1	6	n.d.	4.00	2.85	100
	12	n.d.	4.00	2.97	100
	16	n.d.	4.00	2.92	100
FG16	6	n.d.	4.00	2.82	100
	12	n.d.	4.00	2.96	100
	16	n.d.	4.00	2.96	100

^aMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable.
^bTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-a) × 100.

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