Skip to main content

Table 4 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector during fed-batch culture

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Clone Name Culture period (day) Relative composition of monosaccharide Fucose(-)%b
   Fucosea GlcNAc Mannose  
32-05-12 6 0.92 4.00 2.86 8
  12 0.94 4.00 2.99 6
  16 0.97 4.00 3.01 3
FG1 6 n.d. 4.00 2.85 100
  12 n.d. 4.00 2.97 100
  16 n.d. 4.00 2.92 100
FG16 6 n.d. 4.00 2.82 100
  12 n.d. 4.00 2.96 100
  16 n.d. 4.00 2.96 100
  1. aMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable.
  2. bTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-a) × 100.