Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Table 3 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector

Clone Name	siRNA^a	Relative composition of monosaccharide			Fucose(-)%^c
		Fucose^b	GlcNAc	Mannose
32-05-12	-	0.96	4.00	2.60	4
FG1	+	n.d.	4.00	2.58	100
FG3	+	n.d.	4.00	2.65	100
FG4	+	n.d.	4.00	2.63	100
FG7	+	0.02	4.00	2.57	98
FG9	+	n.d.	4.00	2.61	100
FG13	+	0.02	4.00	2.55	98
FG16	+	n.d.	4.00	2.57	100
FG20	+	n.d.	4.00	2.62	100

^aIntroduction of FUT8 and GMD siRNA tandem expression vector.
^bMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable.
^cTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-b) × 100.

ISSN: 1472-6750