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Table 3 Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Clone Name siRNAa Relative composition of monosaccharide Fucose(-)%c
   Fucoseb GlcNAc Mannose  
32-05-12 - 0.96 4.00 2.60 4
FG1 + n.d. 4.00 2.58 100
FG3 + n.d. 4.00 2.65 100
FG4 + n.d. 4.00 2.63 100
FG7 + 0.02 4.00 2.57 98
FG9 + n.d. 4.00 2.61 100
FG13 + 0.02 4.00 2.55 98
FG16 + n.d. 4.00 2.57 100
FG20 + n.d. 4.00 2.62 100
  1. aIntroduction of FUT8 and GMD siRNA tandem expression vector.
  2. bMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable.
  3. cTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-b) × 100.