BMC Biotechnology

Table 1 Ratio of LCA-resistant clones to drug-resistant clones transformed by the introduction of siRNA expression vectors

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Vector	Target mRNA	Drug^r clones^a	LCA^r clones^b	% LCA^r/Drug^r
FUT8shRNA/lib3/pPUR	FUT8	1344	155	11.5
pPUR/GMDshB	GMD	1440	213	14.8
GFT_3G10/pPUR	GFT	1296	11	0.8
FT8lib3_GMDB/pAGE	GMD·FUT8	1500	730	48.7

^aDrug^r, puromycin resistance or hygromycin resistance.
^bLCA^r, LCA resistance

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ISSN: 1472-6750

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