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Table 1 Ratio of LCA-resistant clones to drug-resistant clones transformed by the introduction of siRNA expression vectors

From: Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Vector Target mRNA Drugr clonesa LCAr clonesb % LCAr/Drugr
FUT8shRNA/lib3/pPUR FUT8 1344 155 11.5
pPUR/GMDshB GMD 1440 213 14.8
GFT_3G10/pPUR GFT 1296 11 0.8
FT8lib3_GMDB/pAGE GMD·FUT8 1500 730 48.7
  1. aDrugr, puromycin resistance or hygromycin resistance.
  2. bLCAr, LCA resistance