Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Enhanced transduction of colonic cell lines in vitroand the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin

Figure 4

Transduction of the inflamed colon in mice by AAV2-microbead conjugates bearing Con A upon intracolonical administration. AAV2-microbead conjugates with and without Con A were prepared at 32 AAV2 particles per microbead, as described in Figs. 3 and 4. These conjugates, along with free, unmodified AAV2.CMV-LacZ, were administered intracolonically into mice with TNBS-induced colitis by enema (a total of 1 × 1010 AAV2 particles in 100 μl PBST per mouse). At 48-hr post-administration, mice were euthanized, and cryosections of their colons were prepared. These colon sections were stained for β-galactosidase activity using X-gal as the substrate, followed by counter-staining with eosin/phloxine B. Stained colon sections were examined under a light microscope. A, free, unmodified AAV2.CMV-LacZ; B, AAV2-microbead conjugates without Con A; C and D, AAV2-microbead conjugates bearing Con A. AC, absorptive cell; GC, goblet cell; LP, lamina propria; ME, muscularis externa; MM, muscularis mucosa; Muc, mucosal layer; SubM, sub-mucosal layer. Representative images are shown.

Back to article page