Skip to main content
Figure 1 | BMC Biotechnology

Figure 1

From: Enhanced transduction of colonic cell lines in vitroand the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin

Figure 1

Infectivity analysis of AAV2 treated with sulfo-NHS-LC-biotin. AAV2.CMV-LacZ was treated with varying concentrations of sulfo-NHS-LC-biotin at room temperature for 45 min in the dark, followed by the addition of excess glycine to terminate the biotinylation reaction. The resulting biotinylated AAV2 preparations were applied to HeLa cells (5 × 106 AAV2 particles per well), which had been grown in 24-well plates at 37°C for 24 hr (initial cell number, 5 × 104 cells per well), and incubated at 37°C for 48 hr. Cells were fixed with glutaraldehyde, stained for β-galactosidase (LacZ) activity using X-gal as the substrate. Then, the number of infected cells, which were stained blue, in each well was counted under a light microscope (--). The same analysis was also performed on biotinylated AAV2 preparations, to which excess Neutralite avidin (100 μg per 5 × 106 AAV2 particles) had been added (--). Each datum shown is the average number of infected cells per well with a standard deviation (n = 26).

Back to article page