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Table 2 Sequences of some anti-tubulin scFvs

From: A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

 

VH CDR3

VL CDR3

    

Name

Sequence

length

Sequence

length

frequencya

yieldb

WBc

IFd

Round 2

        

C12C

SSITIFGGGMDV

12

HSREVHRTF

9

1/5

19

  

E12C

SGGNTFDY

8

QQYYRKPWT

9

1/5

53

  

F1C

GNADGGENWELFDK

14

QLYQNTLWT

9

2/5

52

  

H6C

SSITIFGGGMDV

12

QQNWTSPLS

9

1/5

nd

  

Round 3

        

2C1C

RGRDY

5

QQYNTSPFS

9

1/6

8.6

+

-

2E11C

GRNVLNY

7

QQNSSSPRFT

10

2/6

8.7

+

-

2F12C

GRRALGN

7

QQYNTSPFS

9

1/6

45

+

+

2G4C

GRRALGN

7

LTWSMRSAI

9

1/6

15

+

+

2G9C

GRRALGN

7

LTTENSVYRLV

11

1/6

50

+

-

  1. Sequences of the CDR3s of the best positive clones in an ELISA using cytoplasmically expressed scFv from the 2nd (5 clones) and the 3rd (6 clones) round of selection (Table 2). a Frequency of apparition of the scFv among sequenced clones of the same round. b Yield: mg of scFv purified from 1 liter of cells grown in a flask (OD600 = 5). c WB: detection of tubulin in brain extracts by Western blot. d IF: + means that the scFv is able to reveal microtubule network by Immunofluorescence (Fig. 8). The sequences of the clones 2C1C, 2E11C, 2F12C, 2G4C and 2G9C have been submitted to the EMBL database and their accession numbers are respectively AM886280, AM886281, AM886282, AM886283 and AM886284.