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Table 1 Diversity of the CDR3 libraries

From: A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

  

CAM phenotypeb

 
 

Initial diversitya

++

+

-

final diversityc

H3-5

1.4e8

15/20

3/20

2/20

1.1e8

H3-6

2.7e7

20/20

0/20

0/20

2.7e7

H3-7

9.2e7

16/20

3/20

1/20

7.4e7

H3-8

5.0e6

14/20

4/20

2/20

3.5e6

H3-9

2.4e8

16/20

1/20

3/20

1.9e8

H3-10

1.0e7

10/20

6/20

4/20

5.0e6

H3-11

2.2e7

9/12

1/12

2/12

1.7e7

H3-12

1.0e7

15/20

2/20

3/20

7.5e6

H3-13

2.3e7

8/12

3/12

1/12

1.5e7

H3-14

1.0e7

12/20

4/20

4/20

6.0e6

H3-15

2.1e7

8/12

3/12

1/12

1.4e7

H3-16

1.0e7

12/20

2/20

6/20

6.0e6

H3-17

1.1e7

12/20

4/20

4/20

6.6e6

K3-9

3.6e6

11/16

1/16

4/16

2.5e6

K3-10

4.4e6

10/16

1/16

5/16

2.8e6

L3-9

1.7e7

11/20

6/20

3/20

9.4e6

L3-10

1.5e7

10/20

9/20

1/20

7.5e6

L3-11

1.8e7

15/20

3/20

2/20

1.4e7

  1. a Initial diversity of the library cloned as fusion with CAT and selected on ampicillin. This is the number of clones obtained after transformation.
  2. b Between 12 and 20 clones from the transformation were checked on CAM plates. Plates were incubated for 16 h at 37°C and colony size estimated. Columns ++,+ and - give, respectively, the fraction of clones that grew normally, gave tiny colonies, and did not grow at all.
  3. c Diversity of the libraries selected on CAM. The diversity is estimated from column "Initial Diversity" and "CAM phenotype" by assuming that the final diversity is close to (Initial Diversity) × (CAM phenotype ++). The real diversity may be a higher since some of the clones noted + in column "CAM phenotype" may be present in low abundance.