TY - JOUR AU - Philibert, Pascal AU - Stoessel, Audrey AU - Wang, Wei AU - Sibler, Annie-Paule AU - Bec, Nicole AU - Larroque, Christian AU - Saven, Jeffery G. AU - Courtête, Jérôme AU - Weiss, Etienne AU - Martineau, Pierre PY - 2007 DA - 2007/11/22 TI - A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm JO - BMC Biotechnology SP - 81 VL - 7 IS - 1 AB - Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody fragments cannot be used as intrabodies because they do not fold under the reducing conditions of the cell cytosol and nucleus. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-7-81 DO - 10.1186/1472-6750-7-81 ID - Philibert2007 ER -