Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

Table 2 The bacterial strains and plasmids used in this work

Strain/Plasmid	Genotype/accession number	Source	Reference
Strains
Escherichia coli K-12 MG1655	Wild-type	The All-Russian Collection of Industrial Microorganisms (VKPM)	[35]
E. coli K-12 TG1	Δ(lac-pro) supE thi hsdD5 F'traD36 proAB lacI^q lacZΔM15	VKPM collection	[36]
E. coli K12 C600	F^- e14^- (McrA^-) thi-1 leuB6 lacY1 fhuA21 glnV44 rfbD1	VKPM collection	[37]
E. coli K12 MG1655dam^-	dam13::Tn9(Cm)	This work
E. coli K12 GM2163	F-ara14 leuB6 tonA31 lacY1 tsx78 supE44 galK2 galT22 hisG4 rpsL136 xyl5 mlt1 thi1 dam13::Tn9(Cm) dsm6 hsdR2 mcrB1 mcrA	VKPM collection
E. coli K12 BW25113	lacI^q rrnB_T14 ΔlacZ_WJ16 hsdR514 ΔaraBAD_AH33 ΔrhaBAD_LD78	Prof. B.L. Wanner (personal communications)	[23]
P. ananatis SC17	A low phlegm-producing mutant strain	Ajinomoto Co	[28]
Plasmids
RSF1010	GenBank accession number NC_001740	Ajinomoto Co	[7]
pBluescript II SK(+)	GenBank accession number X52328	Stratagene
pMW-P_lacUV5-lacI-118		From the laboratory collection	[19]
PACYC184	GenBank accession number X06403	Fermentas
RP1.2		Drs. E.G. Abalakina and J.V. Jomantas (personal communications)	[25]
pKD46	GenBank accession number AY048746	Prof. B.L. Wanner (personal communications)	[23]

ISSN: 1472-6750