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Table 1 Relative copy numbers of the constructed mob- derivatives of the RSF1010 plasmid

From: Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

Plasmid

Relative copy number

Sm resistance, mg/L

 

CT = 16 h

CT = 3 h

 
  

0 mM IPTG

1 mM IPTG

 

RSF1010

1.0 ± 0.3

1.0 ± 0.1

1.0 ± 0.1

450

RSF1010mob

0.9 ± 0.1

0.6 ± 0.1

1.4 ± 0.1

250

RSF1010mob-I

2.6 ± 0.3

1.8 ± 0.2

2.0 ± 0.1

700

  1. Copy number of RSF1010 plasmid was taken as 1.0. Streptomycin resistance of the plasmid strains was determined on LB agar plates containing different concentrations of the antibiotic in 16 hours incubation at +37°C.