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Figure 3 | BMC Biotechnology

Figure 3

From: Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

Figure 3

Dramatic improvement of Cy5 fluorescence stability as a result of ozone depletion. Pairs of microarrays printed in-house [A] or printed by Agilent Technologies [B] were hybridized and initially scanned immediately after washing in the carbon-filtered lab. One of each of the pairs of slides was then moved to an environment in which ozone was not removed by carbon filtration (ozone ~25 ppb for the in-house produced slide and ~10 ppb for the Agilent slide). The remaining slide remained in the ozone controlled microarray laboratory (ozone ~2–4 ppb). The slides were then alternately rescanned 6 times. The data show the rapid decline in Cy5 feature intensities as early as 13 minutes in the non-ozone controlled environment. It should be noted that the "ozone-exposed" microarray was not exposed to ozone while it was being scanned.

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