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Figure 2 | BMC Biotechnology

Figure 2

From: Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

Figure 2

Reduction of Cy5 signal for in-house fabricated microarrays kept in a none-ozone controlled environment. These figures show a region of two 20 K mouse microarrays, selected because of their wide range of both Cy5 and Cy3 signals. The microarray images were from scans made during a 114 minute experiment interval. While the microarrays maintained in the carbon-filtered laboratory environment (ozone ~2–4 ppb) remained relatively unchanged with time (top images), reduction in Cy5 in the uncontrolled ozone environment (ozone ~25 ppb) causes the microarray image to have a predominantly green cast (bottom images).

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