Figure 2
From: Comparison of chicken 7SK and U6 RNA polymerase III promoters for short hairpin RNA expression
Detection of shEGFP expression from ch7SK-shEGFP expression constructs in DF-1 cells. DF-1 cells were transfected with shEGFP expression constructs as indicated above each lane. RNA samples were probed in solution with 32P-labelled shEGFP-specific LL91 RNA probe [14] and treated with RNAse A/T1. Protected shEGFP fragments were distinguished by comparison to RNA size markers (Decade™, Ambion).