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Table 1 Randomized oligonucleotide primers used for amplification of randomized spNAR V CDR3 sequences.

From: Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library

Direction

Number

Sequence (5'-3')

Forward

Primer mixa

#8406, #8407/8, #7554/6, #9686/7, forextra

Reverse

Primer mixb

#6974/7, 7553/5, #9688/9, revextra

Reverse

spCDR3 R18

GGTYARWRSGGTKCCAKYTCC(MNN)18AGYKTTGCARWWAKACGTGGC

Reverse

spCDR3 R16

GGTYARWRSGGTKCCAKYTCC(MNN)16AGYKTTGCARWWAKACGTGGC

Reverse

spCDR3 R13

GGTYARWRSGGTKCCAKYTCC(MNN)13AGYKTTGCARWWAKACGTGGC

  1. a, b were described in detail as in Liu et al. [20].
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BMC Biotechnology

ISSN: 1472-6750

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