Table 2 Overall mutation rates of DNA-shuffling and RD-PCR
Source | Protocol | Overall mutagenic rate % | Total base pairs sequenced |
|---|---|---|---|
mRFP and DsRed | RD-PCR | 0.02 | 33900 |
mRFP and GFP | RD-PCR | 0.04 | 25200 |
mRFP and DsRed | DNA-shuffling | 0.03 | 21018 |
mRFP and GFP | DNA-shuffling | 0.02 | 8136 |