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Figure 3 | BMC Biotechnology

Figure 3

From: Revealing biases inherent in recombination protocols

Figure 3

Statistical analysis of the libraries generated by each protocol for DsRed/mRFP and GFP/mRFP. Useful sequences are sequences that would be interesting to screen. This measure excludes parental background and does not count duplicate sequences more than once. RD-PCRs 1–4 use one skew primer per parent (Figure 1b), RD-PCR 5 uses two skew primers per parent (Figure 1c). Templates (see Figure 2): RD-PCR1 and RD-PCR 5: full length DsRed/mRFP. RD-PCR 2: DsRed template with the first 5 bp truncated, full length mRFP. RD-PCR 3: DsRed template with 44 base pairs truncated from the 5' end, full length mRFP. RD-PCR 4: Both templates are truncated. RD-PCR 5: with two skew primers per parent.

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