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Figure 9 | BMC Biotechnology

Figure 9

From: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

Figure 9

Amplification plots (Panel A) of GAPDH from various input of cDNA using either EG (open circle) or SG (solid triangle) as the DNA-binding dye in the PCR mix. All reactions were run in duplicates, but only one plot per duplicate is shown for clarity. Plots of Ct value vs. logarithm cDNA input amount for both sets of reactions are shown in panel B. Reactions with EG (open circle) showed earlier Ct values and high amplification efficiency.

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BMC Biotechnology

ISSN: 1472-6750

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