Figure 7

Effect of chain extension time on qPCR using EG or SG: Amplification plots of McG (Panel A1, and A2), TBP (Panel B1 and B2) and GCL (Panel C1 and C2) with EG or SG at 0.5× or 1× dye concentration: 0.5 × EG (dark lines E1–E4 in panels A1, B1 and C1); 1 × EG (dark lines E5–E8 in panels A2, B2 and C2); 0.5 × SG (gray lines S1–S4 in panels A1, B1 and C1); 1 × SG (gray lines S5–S8 in panels A2, B2 and C2). Four different elongation times, i.e., 60 seconds (lines E1, E5, S1 and S5), 30 seconds (lines E2, E6, S2 and S6), 15 seconds (lines E3, E7, S3 and S7) and 5 seconds (lines E4, E8, S4 and S8), were used for the amplification of each gene fragment with each dye and dye concentration. Line trace numbers within parenthesis indicate formation of nonspecific products as confirmed by post PCR DNA melt curve analysis.