Figure 6

Effect of EG and SG concentrations on PCR performance: Amplification plots of McG (Panels A1, and A2), TBP (Panels B1 and B2) and GCL (Panels C1 and C2) using EG (Panels A1, B1 and C1) or SG (Panel A2, B2 and C2) as the DNA-binding dye for real-time fluorescence monitoring. Each template was amplified in duplicate under four different concentrations of each dye: 2× (thick solid line), 1.5× (thick dashed line), 1× (thin solid line), and 0.5× (thin dashed line) (1 × SG = 1.33 μM; 1 × SG = 0.67 μM). At 1.5 × SG and 2 × SG, amplifications of both McG and TBP produced non-specific products as revealed by post-PCR DNA melt curve analysis and agarose gel electrophoresis.