Figure 3
Fluorescence intensity of EG (11.15 μM) in response to titration using single-stranded M13 DNA (circle) and double-stranded λDNA (square), respectively. The linear response range for double stranded DNA titration is from 0 to about 10 ng/μL with a slope of 0.148 while the linear response range for single-stranded DNA titration is from 0 to at least 40 ng/μL with a slope of 0.013. Thus, the ratio of the two slopes is 11.4.