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Figure 2 | BMC Biotechnology

Figure 2

From: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

Figure 2

Excitation spectra of EG free dye (dark dotted line) and EG in the presence of λDNA at 40 ng/μL (dark solid line) and emission spectra of EG free dye (gray dotted line), EG in the presence of λDNA at 40 ng/μL (gray solid line), and EG in the presence of M13 DNA at 40 ng/μL (gray dashed line). The excitation spectra were normalized to the same intensity to better compare the relative wavelength shift while the emission spectra were plotted in relative intensities in order to compare the fluorescence brightness of the dye under different conditions. All spectra were measured at room temperature in AmpliTaq DNA polymerase buffer (ABI) with a dye concentration of 11.42 μM.

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