Figure 10From: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applicationsMelt curves of the GAPDH amplicon in the presence of EG (solid line) or SG (dashed line) following the amplification of 100 pg human cDNA input as described for Figure 9. The melt peak recorded with EG is about 10 times more intense than that recorded with SG while its width is only about one half of the latter at the half-peak height.Back to article page