Table 4 Flow cytometry analysis of two MCF7 and MDA-MB-468 tumor cell lines and normal breast epithelial MCF10-2A cells. Irrelevant antibody anti-SP2 was used as negative control, while an α-tubulin monoclonal antibody was used as positive control for intracellular staining

α-tubulin

n.t.

n.t.

n.t.

88.04

684.49

60262.5

anti-SP2

2.73

24.45

66.7

0.36

4221.17

1519.6

MIX7

2.84

29.19

82.9

73.03

603.58

44079.4

MIX17

2.68

22.76

59.9

84.2

733.63

61771.6

MIX39

3.98

22.9

91.1

13.89

504.85

7012.4

MDA-MB-468

% pos

MFI

iMFI

% pos

MFI

iMFI

α-tubulin

n.t.

n.t.

n.t.

85.22

259.18

22087.3

anti-SP2

1.9

51.17

97.2

0.98

579.13

567.5

MIX7

3.93

40.4

158.8

37.14

214.39

7962.4

MIX17

5.38

33.04

177.8

70.54

260.36

18365.8

MIX39

3.33

30.04

100.0

2.51

237.64

596.5

MCF10-2A

% pos

MFI

iMFI

% pos

MFI

iMFI

α-tubulin

n.t.

n.t.

n.t.

42.05

169.64

7133.4

anti-SP2

0.44

95.68

42.1

0.44

1515.5

666.8

MIX7

0.94

208.51

196.0

0.29

962.79

279.2

MIX17

0.81

11.84

9.6

1.06

824.83

874.3

MIX39

0.93

175.42

163.1

6.49

156.57

1016.1