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Table 4 Flow cytometry analysis of two MCF7 and MDA-MB-468 tumor cell lines and normal breast epithelial MCF10-2A cells. Irrelevant antibody anti-SP2 was used as negative control, while an α-tubulin monoclonal antibody was used as positive control for intracellular staining

From: Tumor-infiltrating B lymphocytes as an efficient source of highly specific immunoglobulins recognizing tumor cells

Cell line/scFv antibody Surface staining of alive cells Intracellular staining
MCF7 % pos MFI* iMFI** % pos MFI iMFI
α-tubulin n.t. n.t. n.t. 88.04 684.49 60262.5
anti-SP2 2.73 24.45 66.7 0.36 4221.17 1519.6
MIX7 2.84 29.19 82.9 73.03 603.58 44079.4
MIX17 2.68 22.76 59.9 84.2 733.63 61771.6
MIX39 3.98 22.9 91.1 13.89 504.85 7012.4
MDA-MB-468 % pos MFI iMFI % pos MFI iMFI
α-tubulin n.t. n.t. n.t. 85.22 259.18 22087.3
anti-SP2 1.9 51.17 97.2 0.98 579.13 567.5
MIX7 3.93 40.4 158.8 37.14 214.39 7962.4
MIX17 5.38 33.04 177.8 70.54 260.36 18365.8
MIX39 3.33 30.04 100.0 2.51 237.64 596.5
MCF10-2A % pos MFI iMFI % pos MFI iMFI
α-tubulin n.t. n.t. n.t. 42.05 169.64 7133.4
anti-SP2 0.44 95.68 42.1 0.44 1515.5 666.8
MIX7 0.94 208.51 196.0 0.29 962.79 279.2
MIX17 0.81 11.84 9.6 1.06 824.83 874.3
MIX39 0.93 175.42 163.1 6.49 156.57 1016.1
  1. * mean fluorescence intensity
  2. ** integrated MFI (% pos × MFI)
  3. n.t.- not tested