Fluorescence staining and flow cytometry with anti-MUC1 and anti-CEA antibodies. (a)Fluorescence staining of breast carcinoma cells MCF7 and SkBr3, expressing epithelial mucin MUC1 and normal breast epithelial MCF10-2A cells by using phage antibody anti-MUC1 MB5 (left panels). Right panels show results of flow cytometry analysis of phage displayed MB5 and irrelevant anti-SP2 single-chain antibodies. (b)Staining of LoVo colorectal adenocarcinoma cells expressing CEA protein by phage-displayed anti-CEA CB37 scFv antibody is shown. Staining of negative control MCF10-2A cells is included (left panels). Binding of phage antibody CB37 to LoVo was also measured by flow cytometry (right panels).