Selection of anti-MCF7 antibodies. (a)Reactivity of phage pools after fourth and fifth rounds of panning, in comparison with original libraries, was tested. Data reported are the average values of assays performed in triplicate. (b)Fingerprinting analysis of antibody clones. PCR-amplified scFv genes were analyzed by using HaeIII and AluI double digestion. The analysis of clones 17–39, selected from mixLIB, is shown at right, and the list of different anti-MCF7 antibodies obtained shown at left. (c)Cell ELISA reactivity of single phage clones. Data reported are the average values of assays performed in triplicate. Cells developing with irrelevant anti-SP2 antibody are included as negative control.