Figure 5From: Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domainsHeat tolerance of the VH soluble domain C47. The protein was denatured by a heat treatment (20 min × 70°C) and then left to recover 30 min on ice. CD spectra of native, heat-unfolded and refolded C47 were recovered and the aggregation indexes were measured at the fluorimeter.Back to article page