Figure 2From: Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domainsRE3 VHH binding capacity is not modified by heat treatment. ELISA was performed using samples of both heat-treated and control RE3, an unspecific VHH directed against lysozyme as a control, and in the presence of the only secondary antibody directed against the His-tag.Back to article page