Figure 2

Detection of RNA on solid support. Solid support detection of in vitro transcribed RNA with Turtle Probes. (A) Detection of the HPV16E6a RNA with the probe TP-polyA-id33. (B) Detection of the SSA4-3'UTR RNA fragment with the probe TP-SSA4end-id16. (C) Combined detection of the HPV16E6a RNA and the SSA4-3'UTR RNA fragment with the probes TP-polyA-id33 (green) and TP-SSA4end-id16 (red). The two probes were co-hybridized, co-amplified, and co-detected with a mixture of Lin16 (red identifier) and Lin33 (green identifier). (D-F) Negative controls as (A) save for the following: (D) No capture oligonucleotide present; (E) No RNA was added; (F) The RNA added lacked the artificial polyA tail (HPV16E6noPA RNA) but otherwise had the same sequence as the HPV16E6a RNA used in (A). A 63× objective was used and scale bar is 50 μm.