Table 3 Validation of the proposed method on ten randomly selected nuclei images
From: High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles
Image | # of nuclei (manual counted) | # of nuclei (correctly – segmented) | # of nuclei (over – segmented) | # of nuclei (under – segmented) | # of nuclei (missed) | # of nuclei (noises) |
|---|---|---|---|---|---|---|
1 | 858 | 831 (96.8%) | 10 (1.2%) | 2 (0.2%) | 15 (1.7%) | 42 (4.9%) |
2 | 1082 | 1030 (95.1%) | 30 (2.8%) | 15 (1.4%) | 7 (0.6%) | 40 (3.7%) |
3 | 671 | 653 (97.3%) | 12 (1.8%) | 5 (0.7%) | 1 (0.1%) | 41 (6.1%) |
4 | 900 | 867 (96.3%) | 21 (2.3%) | 7 (0.8%) | 5 (0.6%) | 55 (6.1%) |
5 | 723 | 700 (96.8%) | 17 (2.4%) | 3 (0.4%) | 3 (0.4%) | 41 (5.7%) |
6 | 750 | 712 (94.9%) | 26 (3.5%) | 5 (0.7%) | 7 (0.9%) | 54 (7.2%) |
7 | 836 | 801 (95.8%) | 23 (2.8%) | 6 (0.7%) | 6 (0.7%) | 41 (4.9%) |
8 | 720 | 679 (94.3%) | 34 (4.7%) | 4 (0.6%) | 3 (0.4%) | 43 (6.0%) |
9 | 852 | 811 (95.2%) | 20 (2.3%) | 8 (0.9%) | 13 (1.5%) | 53 (6.2%) |
10 | 773 | 746 (96.5%) | 16 (2.1%) | 7 (0.9%) | 4 (0.5%) | 55 (7.1%) |
Avg. | 817 | 783 (95.9%) | 21 (2.6%) | 6 (0.7%) | 6 (0.8%) | 47 (5.7%) |