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Table 1 Values and description of the parameters used in the proposed method

From: High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles

Parameter Value Description
c b 3 Threshold for bright nuclei binarization
c d 0.3 Threshold for dark nuclei binarization
σ b 2.5 Sigma of Gaussian filtering for bright nuclei
σ d 5 Sigma of Gaussian filtering for dark nuclei
T b 50 pixels Threshold of central point detection of bright nuclei
T d 100 pixels Threshold of central point detection of dark nuclei
r b 1 3 D b MathType@MTEF@5@5@+=feaafiart1ev1aqatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaadaWcaaqaaiabigdaXaqaaiabiodaZaaacqWGebardaWgaaWcbaGaemOyaigabeaaaaa@312B@ Radius of local suppression of bright nuclei; D b is the diameter of bright nuclei (D b ≈ 9.675 micron).
r d 1 3 D d MathType@MTEF@5@5@+=feaafiart1ev1aqatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaadaWcaaqaaiabigdaXaqaaiabiodaZaaacqWGebardaWgaaWcbaGaemizaqgabeaaaaa@312F@ Radius of local suppression of bright nuclei; D d is the diameter of dark nuclei (D d ≈ 19.35 micron).
T pdf e -41 Threshold of the PDF score