Table 1 Values and description of the parameters used in the proposed method
From: High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles
Parameter | Value | Description |
|---|---|---|
c b | 3 | Threshold for bright nuclei binarization |
c d | 0.3 | Threshold for dark nuclei binarization |
σ b | 2.5 | Sigma of Gaussian filtering for bright nuclei |
σ d | 5 | Sigma of Gaussian filtering for dark nuclei |
T b | 50 pixels | Threshold of central point detection of bright nuclei |
T d | 100 pixels | Threshold of central point detection of dark nuclei |
r b |
| Radius of local suppression of bright nuclei; D b is the diameter of bright nuclei (D b ≈ 9.675 micron). |
r d |
| Radius of local suppression of bright nuclei; D d is the diameter of dark nuclei (D d ≈ 19.35 micron). |
T pdf | e -41 | Threshold of the PDF score |