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Table 1 Amino acid sequence diversity of proteomes and libraries

From: Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library

  Average diversity per position Diversity per 12-mer Functional diversity (# of equally probable sequences)
Proteomes:    
Human 0.85 0.14 5.73 × 1014
E. coli 0.82 0.09 3.69 × 1014
Phage-displayed libraries:    
T7 Trinuc 0.91 0.32 1.31 × 1015
T7 NNK 0.85 0.14 5.73 × 1014
M13 NNK 0.68 0.01 4.10 × 1013
In silico libraries:    
20 codon (trinucleotide) 1.00 1.00 4.10 × 1015
32 codon (reduced) 0.83 0.11 4.51 × 1014
61 codon (standard) 0.79 0.06 2.46 × 1014
  1. Populations of random 12-mer peptides from the human and E. coli proteomes and from computationally-random in silico libraries were collected as previously described and were subjected to diversity analysis by the DIVAA program of the RELIC web server [4]. DIVAA-generated positional diversity estimates were utilized to calculate the average positional diversity estimates for the peptide populations. Diversity per 12-mer was determined by raising the average positional diversity to the twelth-power. Functional diversity [6] was estimated by multiplying the diversity per 12-mer by the total number of possible sequences (2012 for a 12-mer peptide population). Portions of this table were reproduced with kind permission from Wiley-VCH Verlag GmbH & Co. KGaA, see acknowledgements section for details.