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Table 1 Subset of 87 clones tested in E. coli in vitro and in wheat germ expression. ORF Nr.: Clone identifier; RZPD Clone ID: Available clones at RZPD GmbH; Hit Acc. Nr.: Best BLAST hit of DNA sequence. Molecular weight was calculated by translation of the DNA sequence. Expression/solubility were assigned values from 0 (no expression/no protein detectable in the supernatant) to 4 (very strong band/protein band in the supernatant is stronger than in the pellet). The column 'yielded by' is indicated as follows: WG C: wheat germ C-terminal, WG: wheat germ, C- and N-terminal, WG N: wheat germ, N-terminal, RTS CW: E. coli in vitro C-terminal wildtype, RTS CM: E. coli in vitro C-terminal mutant, RTS NW: E. coli in vitro N-terminal wildtype, RTS Pl: E. coli in vitro original plasmid.

From: A systematic approach for testing expression of human full-length proteins in cell-free expression systems

ORF Nr

RZPDCloneID

Hit Acc No

Gene symbol

Mw in kDa

Best expr. rate

Yielded by

Best solubility

264

RZPDo834B052

NM_001677

ATP1B1

36

4

RTS NW

3

400

RZPDo834D022

NM_002573

PAFAH1B3

27

4

WG, RTS CM NW

4

433

 

NM_000318

PXMP3

32

4

WG, RTS CW CM NW

4

464

RZPDo834F012

NM_006793

PRDX3

29

4

WG C, RTS CM

4

505

 

NM_003187

TAF9

30

4

WG

3

531

 

NM_012222

MUTYH

23

4

WG

3

562

RZPDo834B043

NM_000075

CDK4

35

4

RTS CM NW Pl

3

571

RZPDo834B083

NM_003182

TAC1

13

4

WG C, RTS CW CM

4

616

RZPDo834D033

NM_000550

TYRP1

61

3

RTS Pl

2

636

 

NM_000612

IGF2

22

4

WG C, RTS CW CM

4

637

RZPDo834E013

NM_015646

RAP1B

22

4

WG, RTS CW CM NW

4

639

 

NM_002512

NME2

14

4

WG N, RTS NW

3

690

RZPDo834F123

NM_006923

SDF2

23

4

WG C, RTS CW CM

4

694

RZPDo834G023

NM_020470

YIF1

32

4

WG N

3

728

RZPDo834E0511

NM_000434

NEU1

46

4

RTS NW

2

741

RZPDo834H073

NM_002799

PSMB7

30

4

WG C

4

772

RZPDo834C0311

NM_002804

PSMC3

46

4

WG N, RTS CM

4

777

RZPDo834C0411

NM_003908

EIF2S2

39

4

WG

4

831

 

NM_004394

DAP

12

3

WG

4

832

 

NM_002966

S100A10

12

4

WG, RTS CM NW

4

833

RZPDo834A124

NM_017503

SURF2

30

4

WG C

4

840

RZPDo834B024

NM_005499

UBA2

72

3

WG C

4

842

 

NM_005942

MOCS1

24

4

WG N, RTS CM

3

855

 

NM_002134

HMOX2

36

4

RTS NW

4

861

RZPDo834C034

NM_006370

VTI1B

27

4

WG N, RTS NW Pl

3

868

RZPDo834C084

NM_005892

FMNL1

53

4

WG, RTS CM NW

3

873

RZPDo834C104

NM_007363

NONO

55

4

WG C

3

881

 

NM_002622

PFDN1

15

4

WG C

4

898

RZPDo834D114

NM_006117

PECI

21

4

WG N, RTS CW

4

901

RZPDo834E024

NM_031263

HNRPK

51

4

RTS CW NW

4

904

 

NM_004401

DFFA

13

4

WG N

4

906

 

NM_006693

CPSF4

31

4

WG C, RTS CM

4

915

RZPDo834F024

M55654

TBP

38

4

WG N, RTS NW Pl

3

918

 

NM_004184

WARS

54

3

WG N

4

921

RZPDo834F064

NM_004309

ARHGDIA

23

4

RTS CM NW

3

924

RZPDo834F094

NM_002861

PCYT2

44

4

WG C

4

930

RZPDo834G014

NM_001551

IGBP1

40

4

WG, RTS Pl

4

932

RZPDo834G034

NM_002070

GNAI2

41

4

WG C, RTS NW Pl

4

935

 

NM_001014835

PAK4

64

2

WG C

3

936

RZPDo834G064

NM_002074

GNB1

38

3

RTS NW

3

939

RZPDo834G074

NM_006321

ARIH2

58

3

WG C, RTS Pl

4

940

RZPDo834G084

NM_013296

GPSM2

55

2

WG C

4

943

RZPDo834G114

NM_001863

COX6B1

11

4

WG, RTS CW CM Pl

4

944

 

NM_004537

NAP1L1

46

4

WG

4

945

RZPDo834H014

NM_152925

RBM12

59

4

WG C

4

947

RZPDo834H034

NM_001017957

OS-9

70

4

WG C

4

1033

 

NM_007317

KIF22

74

0

 

0

1068

 

NM_206900

RTN2

52

4

WG C

4

1082

 

NM_018074

FLJ10374

37

4

WG; RTS CW CM

4

1091

 

NM_001512

GSTA4

26

4

WG

4

1093

RZPDo834A015

NM_001647

APOD

22

4

WG C, RTS CM

4

1101

 

NM_001643

APOA2

12

4

WG N

3

1115

 

NM_007261

CMRF-35H

25

4

WG, RTS CM NW

4

1189

RZPDo834H106

NM_001425

EMP3

19

4

WG

4

1294

RZPDo834A035

NM_014876

KIAA0063

24

4

WG

4

1330

 

NM_002816

PSMD12

53

4

WG C

4

1453

RZPDo834B115

NM_198216

SNRPB

25

4

WG C, RTS CM

3

1454

RZPDo834H0711

NM_006841

SLC38A3

56

4

WG, RTS NW

3

1461

RZPDo834C025

NM_004047

ATP6V0B

22

3

WG C

1

1462

RZPDo834C035

NM_003145

SSR2

21

4

WG C, RTS CM

3

1480

 

NM_000984

RPL23A

18

3

WG

4

1485

RZPDo834D025

NM_014860

SUPT7L

47

4

WG

4

1487

RZPDo834D035

NM_198120

EBAG9

25

4

WG C

3

1533

RZPDo834F075

NM_013300

HSU79274

31

4

WG C

3

1554

RZPDo834G095

NM_001778

CD48

28

4

RTS NW

3

1555

RZPDo834G105

NM_019111

HLA-DRA

29

4

RTS CM

3

1575

RZPDo834H085

NM_004233

CD83

23

4

WG, RTS CM

4

1576

RZPDo834H095

NM_007024

PL6

39

2

WG N

1

1642

 

NM_003490

SYN3

64

2

WG

4

1670

 

NM_006841

SLC38A3

56

4

WG N, RTS NW

3

1734

RZPDo834F0511

NM_001436

FBL

34

4

WG C

4

1736

RZPDo834G0511

NM_004343

CALR

49

4

WG C

4

2066

 

NM_015723

PNPLA8

89

0

 

0

2225

RZPDo834H0221

NM_018127

ELAC2

93

0

 

0

2229

RZPDo834A046

NM_00513

REC8L1

63

3

WG C, RTS CM

4

2504

 

NM_199053

FLJ12716

65

4

RTS CW CM NW

3

2724

 

NM_173157

NR4A1

65

4

WG N

2

2871

 

NM_018099

MLSTD1

60

4

RTS CW CM NW

3

2938

RZPDo834H0421

NM_015072

TTLL5

92

0

 

0

2949

RZPDo834E1121

NM_020748

KIAA1287

135

0

 

0

2959

 

NM_021932

RIC8

17

4

WG, RTS Pl

4

2962

RZPDo834H0621

NM_014149

HSPC049

78

2

RTS CM NW

0

2964

RZPDo834F1121

NM_003263

TLR1

91

4

RTS CM NW

4

2968

RZPDo834G0821

NM_001040428

SPATA7

65

4

RTS CW CM NW

3

2973

 

NM_032292

FLJ20203

91

4

RTS CW CM NW

3

2978

RZPDo834H0721

NM_014585

SLC40A1

63

0

 

0

2979

RZPDo834E0821

NM_013277

RACGAP1

71

4

RTS CM

0