Skip to main content

Table 1 Subset of 87 clones tested in E. coli in vitro and in wheat germ expression. ORF Nr.: Clone identifier; RZPD Clone ID: Available clones at RZPD GmbH; Hit Acc. Nr.: Best BLAST hit of DNA sequence. Molecular weight was calculated by translation of the DNA sequence. Expression/solubility were assigned values from 0 (no expression/no protein detectable in the supernatant) to 4 (very strong band/protein band in the supernatant is stronger than in the pellet). The column 'yielded by' is indicated as follows: WG C: wheat germ C-terminal, WG: wheat germ, C- and N-terminal, WG N: wheat germ, N-terminal, RTS CW: E. coli in vitro C-terminal wildtype, RTS CM: E. coli in vitro C-terminal mutant, RTS NW: E. coli in vitro N-terminal wildtype, RTS Pl: E. coli in vitro original plasmid.

From: A systematic approach for testing expression of human full-length proteins in cell-free expression systems

ORF Nr RZPDCloneID Hit Acc No Gene symbol Mw in kDa Best expr. rate Yielded by Best solubility
264 RZPDo834B052 NM_001677 ATP1B1 36 4 RTS NW 3
400 RZPDo834D022 NM_002573 PAFAH1B3 27 4 WG, RTS CM NW 4
433   NM_000318 PXMP3 32 4 WG, RTS CW CM NW 4
464 RZPDo834F012 NM_006793 PRDX3 29 4 WG C, RTS CM 4
505   NM_003187 TAF9 30 4 WG 3
531   NM_012222 MUTYH 23 4 WG 3
562 RZPDo834B043 NM_000075 CDK4 35 4 RTS CM NW Pl 3
571 RZPDo834B083 NM_003182 TAC1 13 4 WG C, RTS CW CM 4
616 RZPDo834D033 NM_000550 TYRP1 61 3 RTS Pl 2
636   NM_000612 IGF2 22 4 WG C, RTS CW CM 4
637 RZPDo834E013 NM_015646 RAP1B 22 4 WG, RTS CW CM NW 4
639   NM_002512 NME2 14 4 WG N, RTS NW 3
690 RZPDo834F123 NM_006923 SDF2 23 4 WG C, RTS CW CM 4
694 RZPDo834G023 NM_020470 YIF1 32 4 WG N 3
728 RZPDo834E0511 NM_000434 NEU1 46 4 RTS NW 2
741 RZPDo834H073 NM_002799 PSMB7 30 4 WG C 4
772 RZPDo834C0311 NM_002804 PSMC3 46 4 WG N, RTS CM 4
777 RZPDo834C0411 NM_003908 EIF2S2 39 4 WG 4
831   NM_004394 DAP 12 3 WG 4
832   NM_002966 S100A10 12 4 WG, RTS CM NW 4
833 RZPDo834A124 NM_017503 SURF2 30 4 WG C 4
840 RZPDo834B024 NM_005499 UBA2 72 3 WG C 4
842   NM_005942 MOCS1 24 4 WG N, RTS CM 3
855   NM_002134 HMOX2 36 4 RTS NW 4
861 RZPDo834C034 NM_006370 VTI1B 27 4 WG N, RTS NW Pl 3
868 RZPDo834C084 NM_005892 FMNL1 53 4 WG, RTS CM NW 3
873 RZPDo834C104 NM_007363 NONO 55 4 WG C 3
881   NM_002622 PFDN1 15 4 WG C 4
898 RZPDo834D114 NM_006117 PECI 21 4 WG N, RTS CW 4
901 RZPDo834E024 NM_031263 HNRPK 51 4 RTS CW NW 4
904   NM_004401 DFFA 13 4 WG N 4
906   NM_006693 CPSF4 31 4 WG C, RTS CM 4
915 RZPDo834F024 M55654 TBP 38 4 WG N, RTS NW Pl 3
918   NM_004184 WARS 54 3 WG N 4
921 RZPDo834F064 NM_004309 ARHGDIA 23 4 RTS CM NW 3
924 RZPDo834F094 NM_002861 PCYT2 44 4 WG C 4
930 RZPDo834G014 NM_001551 IGBP1 40 4 WG, RTS Pl 4
932 RZPDo834G034 NM_002070 GNAI2 41 4 WG C, RTS NW Pl 4
935   NM_001014835 PAK4 64 2 WG C 3
936 RZPDo834G064 NM_002074 GNB1 38 3 RTS NW 3
939 RZPDo834G074 NM_006321 ARIH2 58 3 WG C, RTS Pl 4
940 RZPDo834G084 NM_013296 GPSM2 55 2 WG C 4
943 RZPDo834G114 NM_001863 COX6B1 11 4 WG, RTS CW CM Pl 4
944   NM_004537 NAP1L1 46 4 WG 4
945 RZPDo834H014 NM_152925 RBM12 59 4 WG C 4
947 RZPDo834H034 NM_001017957 OS-9 70 4 WG C 4
1033   NM_007317 KIF22 74 0   0
1068   NM_206900 RTN2 52 4 WG C 4
1082   NM_018074 FLJ10374 37 4 WG; RTS CW CM 4
1091   NM_001512 GSTA4 26 4 WG 4
1093 RZPDo834A015 NM_001647 APOD 22 4 WG C, RTS CM 4
1101   NM_001643 APOA2 12 4 WG N 3
1115   NM_007261 CMRF-35H 25 4 WG, RTS CM NW 4
1189 RZPDo834H106 NM_001425 EMP3 19 4 WG 4
1294 RZPDo834A035 NM_014876 KIAA0063 24 4 WG 4
1330   NM_002816 PSMD12 53 4 WG C 4
1453 RZPDo834B115 NM_198216 SNRPB 25 4 WG C, RTS CM 3
1454 RZPDo834H0711 NM_006841 SLC38A3 56 4 WG, RTS NW 3
1461 RZPDo834C025 NM_004047 ATP6V0B 22 3 WG C 1
1462 RZPDo834C035 NM_003145 SSR2 21 4 WG C, RTS CM 3
1480   NM_000984 RPL23A 18 3 WG 4
1485 RZPDo834D025 NM_014860 SUPT7L 47 4 WG 4
1487 RZPDo834D035 NM_198120 EBAG9 25 4 WG C 3
1533 RZPDo834F075 NM_013300 HSU79274 31 4 WG C 3
1554 RZPDo834G095 NM_001778 CD48 28 4 RTS NW 3
1555 RZPDo834G105 NM_019111 HLA-DRA 29 4 RTS CM 3
1575 RZPDo834H085 NM_004233 CD83 23 4 WG, RTS CM 4
1576 RZPDo834H095 NM_007024 PL6 39 2 WG N 1
1642   NM_003490 SYN3 64 2 WG 4
1670   NM_006841 SLC38A3 56 4 WG N, RTS NW 3
1734 RZPDo834F0511 NM_001436 FBL 34 4 WG C 4
1736 RZPDo834G0511 NM_004343 CALR 49 4 WG C 4
2066   NM_015723 PNPLA8 89 0   0
2225 RZPDo834H0221 NM_018127 ELAC2 93 0   0
2229 RZPDo834A046 NM_00513 REC8L1 63 3 WG C, RTS CM 4
2504   NM_199053 FLJ12716 65 4 RTS CW CM NW 3
2724   NM_173157 NR4A1 65 4 WG N 2
2871   NM_018099 MLSTD1 60 4 RTS CW CM NW 3
2938 RZPDo834H0421 NM_015072 TTLL5 92 0   0
2949 RZPDo834E1121 NM_020748 KIAA1287 135 0   0
2959   NM_021932 RIC8 17 4 WG, RTS Pl 4
2962 RZPDo834H0621 NM_014149 HSPC049 78 2 RTS CM NW 0
2964 RZPDo834F1121 NM_003263 TLR1 91 4 RTS CM NW 4
2968 RZPDo834G0821 NM_001040428 SPATA7 65 4 RTS CW CM NW 3
2973   NM_032292 FLJ20203 91 4 RTS CW CM NW 3
2978 RZPDo834H0721 NM_014585 SLC40A1 63 0   0
2979 RZPDo834E0821 NM_013277 RACGAP1 71 4 RTS CM 0