Wheat germ expression in vitro. Presented are western blots of 8 targets expressed with C-terminal (left) and N-terminal (right) 6xHis-tag. ORF Nr.: clone identifier. GUS: glucuronidase is the positive control. Successful protein expression was defined for values 2 – 4 and unsuccessful protein expression for values of 0 and 1. Bands of theexpected size are marked with a +.