Figure 4From: A systematic approach for testing expression of human full-length proteins in cell-free expression systemsE.coli expression in vitro. Presented are western blots of 8 targets expressed with C-terminal wild type, C-terminal mutant, N-terminal wild type and original plasmid DNA templates (from left to right). Successful protein expression was defined for values 2 – 4 and unsuccessful protein expression for values of 0 and 1. Bands of the expected size are marked with a +.Back to article page