Knockdown of Ap-crt-1 expression. Ap-crt-1 mRNA level in control LacZ and dsAp-crt-1 injected aphids were monitored by qRT-PCR over seven days after injection (dpi). Each kinetic point was performed in triplicate on 7–10 pooled aphids. For each sample, Ap-crt-1 transcripts level was normalised against Rpl-7. Normalised Ap-crt-1 expression in ds1Ap-crt-1 aphids was expressed as the proportion of the expression recorded in the Lac-Z control. Average percentages of Ap-crt-1 mRNA depletion are indicated. Developmental status of aphids is indicated below x axes.