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Figure 3 | BMC Biotechnology

Figure 3

From: A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods

Figure 3

Chemotaxis of human breast cancer cells in a concentration gradient (0–50 ng/ml) of EGF. (A) First and last images of cells in the gradient region after 5 hours. (B) Directional plot of migration angle in 3–45 ng/ml EGF gradients, grouped in 10° intervals. (C) Cells inside the dotted lines (3–45 ng/ml EGF) were tracked and analyzed. The EGF gradient profile is visualized by FITC-dextran (10 kD) with a similar molecular weight to EGF. Scale bars are 20 μm.

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