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Figure 1 | BMC Biotechnology

Figure 1

From: Improved single-chain transactivators of the Tet-On gene expression system

Figure 1

TetR-based transactivators. (A) tTA contains the N-terminal transposon Tn10-derived Tet repressor and the C-terminal herpes simplex virus VP16-derived activation domain (AD). The TetR part can be subdivided in a DNA-binding domain (BD) and a regulatory core domain (core). rtTA (rtTA2S-S2 in ref. [6]) is a tTA variant with four amino acid substitutions in the TetR domain (shown above the protein). The mutations that enhance rtTA activity are also located in the TetR domain (shown under the protein). sc-rtTA is a single-chain version of rtTA that contains two TetR moieties connected head to tail by a peptide linker and a single activation domain at the C-terminal end. (B) Both tTA and rtTA are active as homodimers, whereas sc-rtTA folds intramolecularly.

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