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Table 2 Percentage of phage p2 inhibition by bacterially produced VHH

From: Lactobacillli expressing llama VHH fragments neutralise Lactococcusphages

Bacteria

% inactivationa

 

Supernatantb

Bacteriac

L. paracasei parental

1.8 ± 5.9

2.1 ± 1.0

L. paracasei + pLP402

4.6 ± 4.0

-4.4 ± 6.4

L. paracasei + pLP402-VHH5-secreted

86.0 ± 5.1

ND

L. paracasei + pLP402-VHH2-anchored

3.4 ± 6.6

31.4 ± 2.8

  1. a Mean ± standard deviation of at least three experiments
  2. b 10 μl supernatant (approximately 5 ng VHH)
  3. c 4 × 106 bacteria