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Table 1 Binding of the expressed VHH fragments to phage p2 antigens by ELISA

From: Lactobacillli expressing llama VHH fragments neutralise Lactococcusphages

 

Supernatanta

Cell extractb

Dilutionc

pLP402-VHH5-secreted

pLP402

pLP402-VHH2-anchored

pLP402

undiluted

0.987

0.076

1.549

0.084

1/4

0.519

-

1.207

-

1/16

0.339

-

0.511

-

1/64

0.128

-

0.215

-

  1. a Supernatant from a culture grown until an OD600 nm of 0.8.
  2. b Cell extract was made by sonication of 2.5 × 109 CFU in 1 ml of PBS.
  3. c Undiluted or dilutions of supernatant and cell extract were added to the wells.
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BMC Biotechnology

ISSN: 1472-6750

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