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Table 1 Binding of the expressed VHH fragments to phage p2 antigens by ELISA

From: Lactobacillli expressing llama VHH fragments neutralise Lactococcusphages

  Supernatanta Cell extractb
Dilutionc pLP402-VHH5-secreted pLP402 pLP402-VHH2-anchored pLP402
undiluted 0.987 0.076 1.549 0.084
1/4 0.519 - 1.207 -
1/16 0.339 - 0.511 -
1/64 0.128 - 0.215 -
  1. a Supernatant from a culture grown until an OD600 nm of 0.8.
  2. b Cell extract was made by sonication of 2.5 × 109 CFU in 1 ml of PBS.
  3. c Undiluted or dilutions of supernatant and cell extract were added to the wells.