Figure 2
From: Lactobacillli expressing llama VHH fragments neutralise Lactococcusphages
Determination of the expression of VHH fragments by L. paracasei using Western blot assay. (A) lane 1, supernatant of pLP402; lane 2, supernatant of pLP402-VHH5-secreted showing the secreted mature VHH of 23 kDa and a degradation product of 19 kDa; lane 3, cell extracts of pLP402-VHH5-secreted showing the precursor VHH (signal peptide-VHH) of 26 kDa and the mature VHH of 23 kDa. (B) lane 1, cell extracts of pLP401-VHH2-anchored showing a anchored VHH2 of 46 kDa; lane 2, control VHH2-E-tag (supernatant from pLP402-VHH2-secreted); lane 3; cell extracts of pLP402.