Figure 1

Map of the Lactobacillus expression vectors. (A) pLP402-VHH5-secreted with a stop codon (TAA) inserted after the E-tag sequence, mediating secretion of the antibody fragment and (B) pLP401-VHH2-anchored mediating surface-anchored expression of antibody fragments by fusion to the last 244 amino acids of the Lactobacillus casei proteinase P. Pamy, Promotor sequence of the α-amylase gene of L. amylovorus; SS amy, signal sequence of the α-amylase gene of Lactobacillus amylovorus (36 amino acids); N-terminus amy, N-terminus (26 amino acids) of the α-amylase of L. amylovorus; deleted Tldh, remaining sequence after deletion of transcription terminator of the lactate dehydrogenase gene of L. casei; VHH5, VHH fragment against the receptor-binding protein of lactococcal phage p2; VHH2, VHH fragment against the major structural capsid protein of phage p2; Anchor, anchor sequence from the proteinase P gene of L. casei (244 amino acids); Tcbh, transcription terminator sequence of the conjugated bile acid hydrolase gene of Lactobacillus plantarum 80; Amp^r, ampicillin-resistance gene; Ery, erythromycin-resistance gene.