Figure 1

Time course of RNA degradation induced by different tissue handling conditions. RNA was prepared from liver sections incubated at room temperature (RT) (circles), 37°C (triangles), or frozen and thawed at room temperature (F/T) (squares). The dotted line represents the linear trendline for the RT incubated sample set. Dashed or solid lines connect the mean values at hourly or semi-hourly time points for the 37°C or F/T handling condition sets, respectively. Symbol shading indicates replicate experiments conducted on different days from independent sources of tissue.