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Table 4 Immobilization of acylases

From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase

Enzymea UPGKbloaded Protein loaded (mg/g of support) % of protein immobilized Activity (UPGKb/g of support) Specific activity (UPGK/mg of immobilized enzyme)c
PGA wt 100 1.8 97 45.2 26.5 ± (0.5)
PGA-αE194K 100 2.8 100 63.4 22.0 ± (0.7)
PGA-βD494K 138 3.0 95 73.2 24.4 ± (0.4)
PGA-βD403K 100 2.2 97 54.6 26.0 ± (0.6)
PGA-2K 139 3.0 97 86.2 28.7 ± (0.8)
PGA-3K 80 3.2 97 54.0 17.4 ± (0.2)
  1. aThe enzymes were immobilized on agarose beads activated with aldehyde groups at pH 10 and 4°C.
  2. bU, μmol of PGK min-1 mg-1.
  3. cActivities of immobilized enzymes are expressed as units (μmol of PGK hydrolyzed min-1 mg-1) per gram of agarose beads.