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Table 1 Forward and reverse primers used for the PCR mutagenesis

From: New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase

D216K for 5'-GGTACGGGAAAATGGAAGTGGAAAGGGCTATTGCCTTTTG-3'
Name Sequence
D216K rev 5'-CAAAAGGCAATAGCCCTTTCCACTTCCATTTTCCCGTACC-3'
E194K for 5'-CCACTATTGCCGTACAAAAGAGTAACTACCCAC-3'
E194K rev 5'-GTGGGTAGTTACTCTTTTGTACGGCAATAGTGG-3'
D403K for 5'-AGGCGGTGCAGGGAAAGAAATCACCAATCCC-3'
D403K rev 5'-GGGATTGGTGATTTCTTTCCCTGCACCGCCT-3'
D494K for 5'-CCAACGACAAGCAAACGTCCTGTGCTTGCC-3'
D494K rev 5'-GGCAAGCACAGGACGTTTGCTTGTCGTTGG-3'
R495K for 5'-CAACGACAAGCGATAAGCCTGTGCTTGCC-3'
R495K rev 5'-GGCAAGCACAGGCTTATCGCTTGTCGTTGG-3'
D216K/G219K for 5'-ACGGGAAAATGGAAGTGGAAAAAGCTATTGCCT-3'
D216K/G219K rev 5'-AGGCAATAGCTTTTTCCACTTCCATTTTCCCGT-3'
N202K/N205K for 5'-CCCACTTAAATTTAAGCAGCAAAAGTCGCAAACAGC-3'
N202K/N205K rev 5'-GCTGTTTGCGACTTTTGCTGCTTAAATTTAAGTGGG-3'