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Table 1 Cloning efficiency when using Sticky RICE method

From: High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virusexpression vectors

Insert size (bp) 5'PO4 on insert Enzyme mixa Reaction time # of colonies % with insertb
770 No Pol/Kin/Lig 30 min 77 ~90%
770 Yes Pol/Lig 30 min 365 ~90%
  1. aPol, 0.25 Units (U) T4 DNA polymerase; Kin, 0.5 U T4 polynucleotide kinase; Lig, 400 U T4 DNA ligase.
  2. bdetermined by restriction endonuclease screening of plasmids (n = 8)