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Table 1 Cloning efficiency when using Sticky RICE method

From: High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virusexpression vectors

Insert size (bp)

5'PO4 on insert

Enzyme mixa

Reaction time

# of colonies

% with insertb

770

No

Pol/Kin/Lig

30 min

77

~90%

770

Yes

Pol/Lig

30 min

365

~90%

  1. aPol, 0.25 Units (U) T4 DNA polymerase; Kin, 0.5 U T4 polynucleotide kinase; Lig, 400 U T4 DNA ligase.
  2. bdetermined by restriction endonuclease screening of plasmids (n = 8)
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BMC Biotechnology

ISSN: 1472-6750

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