Maps of plasmid T-DNAs used in this study. Cauliflower mosaic virus (CaMV) 35S promoter driven versions of the gfp gene, Tomato bushy stunt virus p19 gene or Tobacco mosaic virus (TMV) vector cDNAs were constructed. All plasmids were based on the binary vector pCB301 backbone. T-DNA border sequences not shown in maps. Open boxes represent open reading frames; black block arrows; 35S promoter (35S Pr); black boxes, CaMV 3' terminator sequence; light grey boxes, Tobacco etch virus 5' non-translated leader sequence (L); dark grey box, ribozyme (Rz); bent arrows, locations of TMV subgenomic promoters. The TMV vectors in pJL36 and pJL43 contain the full complement of TMV genes as well as an additional subgenomic promoter. TMV sequences 5' of the multiple cloning site (MCS) are from the U1 strain of TMV. Virus sequences 3' of the MCS are from the U5 strain of TMV. TMV transcripts are processed by a ribozyme to generate authentic TMV 3' ends. The sequence of the MCS in pJL 36 and 43 are presented. Restriction endonuclease recognition sequences are underlined. Because SapI is non-palindromic (GCTCTTC N1/4) both strands of the MCS of pJL 43 are presented for clarity.