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Figure 2 | BMC Biotechnology

Figure 2

From: Site-directed in vitro immunization leads to a complete human monoclonal IgG4λ that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands

Figure 2

Immunoblot screening of peptide arrays denotes the mAb specificity against the Met 55-cored CDR2-like region of human CD152. 1 μg/mL of purified mAb was used to determine the binding epitope. Sequences of EYASPGKATEVRVTV, KVELMYPPPYYLGIG and QYIKANSKFIGITEL indicate CDR1-encompassing region, CDR3-encompassing region and T cell epitope (italic) used for site-directed immunization, respectively.

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