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Figure 9 | BMC Biotechnology

Figure 9

From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Figure 9

Effects of introducing a copy of the 3'-TRD internal domain on leftward gene expression. The genetic structures of the different piggyBac vectors assayed in this experiment (A-E) are shown above the plots in Columns A, B, and C. The dual constitutive (ie1-hr5-ie1) transcriptional control element is indicated by open boxes, the dual inducible (P CMVmin -TetO7-P CMVmin ) transcriptional control element is indicated by horizontally striped boxes, the orientations of the various heterologous genes are shown, and the new 3'-TRD internal domain sequence introduced in the 5'-TRD region is marked with an cross-hatched oval and an arrow to show its orientation. The plot in Column A shows the GalT activities induced by the various GalT-encoding vectors A-E. The plot in Column B shows the ST6GalI activities induced by the various ST6GalI-encoding vectors A-E. The plot in Column C shows the amounts of CMP-sialic acid produced by the various CMP-SAS-encoding vectors A-E. The background levels in each assay were determined using extracts of mock-transfected Sf9 cells and are shown by the bars labeled "None".

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BMC Biotechnology

ISSN: 1472-6750

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