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Figure 7 | BMC Biotechnology

Figure 7

From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Figure 7

Effects of deleting the transposase transcriptional control element in the 5'-TRD region. The genetic structures of the constitutive piggyBac vectors assayed in this experiment (A-D) are shown at the top, with the orientations of the various heterologous genes shown, and the transposase promoter deletion indicated. The rightward-oriented arrow marked with a question mark depicts the hypothesis that antisense transcription originating in the 5'-TRD region could down-regulate expression of the leftward-facing heterologous genes. The plot shows the relative GalT activity levels induced by each of the indicated piggyBac vectors, together with the background levels determined using extracts of mock-transfected Sf9 cells (None).

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BMC Biotechnology

ISSN: 1472-6750

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