Figure 3From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBacpiggyBac vector constructions (continued). PCR amplification of six genes encoding mammalian glycosylation enzymes and their subsequent insertion into the key intermediate plasmids to produce the constitutive (ie1-hr5-ie1) dual piggyBac vectors.Back to article page